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. 2024 Feb 28;11(16):2308637. doi: 10.1002/advs.202308637

Figure 1.

Figure 1

Activated PSCs played a major role in generating the fibrotic stroma of PDAC tissues. A) Activated PSCs (a‐PSCs) and collagen deposition in normal pancreas and PDAC tissues detected by IHC staining for FAP‐α, α‐SMA, and fibronectin, Masson's trichrome staining, and Sirius Red staining. Scale bar, 50 µm, n = 62. B) The uMAP plot shows the cell clusters from 24 PDAC and 11 normal pancreatic tissues. Cell types are coded with different colors. C) Marker genes for different cell clusters. D) Violin plots showing the normalized expression levels of FAP‐α and ACTA2 across cell subpopulations. E) Violin plots showing the normalized expression levels of COL1A1 and COL1A2 across cell subpopulations. F) The correlation between the expression levels of FAP‐α and COL1A1 in PDAC tissues (data from TCGA). G) The correlation between the expression levels of FAP‐α and COL1A2 in PDAC tissues (data from TCGA). H) Immunofluorescence assays showing the protein levels of typical activation markers FAP‐α, α‐SMA, fibronectin, and collagen I in human primary a‐PSCs. Scale bar, 50 µm, n = 3.