|
Antibodies
|
| APC anti-mouse CD25 (clone PC61.5) |
eBioscience |
Cat#17-0251-82; RRID: AB_469366 |
| APC anti-mouse CD11c (clone N418) |
eBioscience |
Cat#17-0114-82; RRID: AB_469346 |
| APC anti-mouse Granzyme B (clone QA16A02) |
Biolegend |
Cat#372204; RRID: AB_2687028 |
| APC anti-mouse T-bet (clone 4B10) |
Biolegend |
Cat#644814; RRID: AB_10901173 |
| R718 anti-mouse Bcl6 (clone K112-91) |
BD Biosciences |
Cat#567413; RRID: AB_2869985 |
| APC/Cyanine7 anti-mouse TCRβ (clone H57-597) |
Biolegend |
Cat#109220; RRID: AB_893624 |
| APC/Cyanine7 anti-mouse B220 (clone RA2-6B2) |
Biolegend |
Cat#103224; RRID: AB_313007 |
| APC/Cyanine7 anti-mouse CD90.2 (clone 53.21) |
BD Biosciences |
Cat#561641; RRID: AB_10898013 |
| APC/Cyanine7 anti-mouse NK1.1 (clone PK136) |
Biolegend |
Cat#108724; RRID: AB_830871 |
| APC/eFlour 780 anti-mouse CD11b (clone M1/70) |
Invitrogen |
Cat#47-0112-82; RRID: AB_1603193 |
| APC/Fire 750 anti-mouse FR4 (clone 12A5) |
Biolegend |
Cat#125013; RRID: AB_2721484 |
| PE anti-mouse FoxP3 (clone FJK-16S) |
eBioscience |
Cat#12-5773-82; RRID: AB_465936 |
| PE anti-mouse I-Ak (clone 10-3.6) |
Biolegend |
Cat#109908; RRID: AB_313457 |
| PE anti-mouse LPAM-1 (α4β7) (clone DATK32) |
Biolegend |
Cat#120605; RRID: AB_493268 |
| PE anti-mouse TNFα (clone MP6-XT22) |
BD Biosciences |
Cat#554419; RRID: AB_395380 |
| PE anti-mouse CCL5 (clone 2E9) |
Biolegend |
Cat#149103; RRID: AB_2564405 |
| PE anti-mouse Gata3 (clone TWAJ) |
eBioscience |
Cat#12-9966-42; RRID: AB_1963600 |
| PE anti-mouse CD103 (clone M290) |
BD Biosciences |
Cat#557495; RRID: AB_396732 |
| PE Rat anti-mouse CD8α |
Biolegend |
Cat#100708; RRID: AB_312747 |
| PE/Dazzle 594 anti-mouse CD215 (IL-15Rα) (clone 6B4C88) |
Biolegend |
Cat#153511; RRID: AB_2922479 |
| PE/FireTM 640 anti-mouse Lag3 (clone C9B7W) |
Biolegend |
Cat#125247; RRID: AB_2924464 |
| PE/Cyanine5 anti-mouse CD11c (clone N418) |
Biolegend |
Cat#117316; RRID: AB_493566 |
| PE/Cyanine7 anti-mouse CD199 (CCR9) (clone CW-1.2) |
Biolegend |
Cat#128711; RRID: AB_10901176 |
| PE/Cyanine7 anti-mouse CD45.1 (clone A20) |
eBioscience |
Cat#25-0453-82; RRID: AB_469629 |
| PE/Cyanine7 anti-mouse CD45 (clone 30-F11) |
eBioscience |
Cat#25-0451-82; RRID: AB_2734986 |
| PE/Cyanine7 anti-mouse IL-12p40 (clone C17.8) |
eBioscience |
Cat#25-7123-82; RRID: AB_2543528 |
| PE/Cyanine7 anti-mouse CD11c (clone N418) |
eBioscience |
Cat#25-0114-82; RRID: AB_469590 |
| PE/Cyanine7 anti-mouse CD73 (clone TY/11.8) |
Biolegend |
Cat#127223; RRID: AB_2716102 |
| FITC anti-mouse Vβ8.1/8.2 (clone KJ-16) |
Biolegend |
Cat#118406; RRID: AB_1227786 |
| FITC anti-mouse CD4 (clone RM4-5) |
Biolegend |
Cat#100510; RRID: AB_312713 |
| FITC anti-mouse Vβ6 (clone RR4-7) |
BD Biosciences |
Cat#553193; RRID: AB_394700 |
| Spark BlueTM 550 anti-mouse CD8α (clone 53-6.7) |
Biolegend |
Cat#100779; RRID: AB_2832268 |
| PerCP/eFluorTM710 anti-mouse I-Ab (MHCII) (clone M5/114) |
eBioscience |
Cat#46-5321-82; RRID: AB_1834439 |
| PerCP/eFluorTM710 anti-mouse Vβ5.1/5.2 (clone MR9-4) |
eBioscience |
Cat#46-5796-82; RRID: AB_10853817 |
| PerCP/Cyanine5.5 anti-mouse CD90.1 (clone OX-70) |
Biolegend |
Cat#202516; RRID: AB_961437 |
| BV421 anti-mouse Vβ4 (clone KT4) |
BD Biosciences |
Cat#743019; RRID: AB_2741216 |
| BV421 anti-mouse CD103 (clone OX-62) |
BD Biosciences |
Cat#566297; RRID: AB_2739670 |
| BV421 anti-mouse TNFα (clone MP6-XT22) |
BD Biosciences |
Cat#563387; RRID: AB_2738173 |
| BV421 anti-mouse CD45 (clone 30-F11) |
BD Biosciences |
Cat#563890; RRID: AB_2651151 |
| BV421 anti-mouse RORγT (clone Q31-378) |
BD Biosciences |
Cat#562894; RRID: AB_2687545 |
| BV421 anti-mouse CCR7 (clone 4B12) |
BD Biosciences |
Cat#562675; RRID: AB_2737716 |
| Pacific BlueTM Anti-rabbit IgG |
Invitrogen |
Cat#P-10994; RRID: AB_2539814 |
| BV480 anti-mouse CD4 (GK1.5) |
BD Biosciences |
Cat#746475; RRID: AB_2743777 |
| BV510 anti-mouse CD62L (clone MEL-14) |
Biolegend |
Cat#104441; RRID: AB_2561537 |
| BV570 anti-mouse CD44 (clone IM7) |
Biolegend |
Cat#103037; RRID: AB_10900641 |
| BV605 anti-mouse CD8α (clone 53-6.7) |
Biolegend |
Cat#100744; RRID: AB_2562609 |
| BV605 anti-mouse CD4 (clone RM4-5) |
BD Biosciences |
Cat#563151; RRID: AB_2687549 |
| BV605 anti-mouse IFNγ XMG1.2) |
Biolegend |
Cat#505839; RRID: AB_2561438 |
| BV605 anti-mouse CD86 (GL-1) |
Biolegend |
Cat#105037; RRID: AB_11204429 |
| BV711 anti-mouse TCRβ (H57-597) |
Biolegend |
Cat#109243; RRID: AB_2629564 |
| BV711 anti-mouse B220 (clone RA2-6B2) |
Biolegend |
Cat#103255; RRID: AB_2563491 |
| BV711 anti-mouse CD80 (clone 16-10A) |
Biolegend |
Cat#104743; RRID: AB_2810338 |
| BV785 anti-mouse PD-1 (clone 29F.1A12) |
Biolegend |
Cat#135225; RRID: AB_2563680 |
| BV785 anti-mouse CD8α (clone 53-6.7) |
Biolegend |
Cat#100749; RRID: AB_11218801 |
| BV786 anti-mouse CD45 (clone 30-F11) |
BD Biosciences |
Cat#564225; RRID: AB_2716861 |
| BV786 Streptavidin |
BD Biosciences |
Cat#563858; RRID: AB_2869529 |
| AF488TM anti-mouse CD11c (clone N418) |
eBioscience |
Cat#53-0114-82; RRID: AB_469903 |
| AF488TM anti-mouse F4/80 (clone BM8) |
Biolegend |
Cat#123120; RRID: AB_893479 |
| AF488TM anti-mouse Anxa2 (clone EPR13052) |
Abcam |
Cat#Ab200791; RRID: AB_2938814 |
| AF647TM anti-mouse CD11b (clone M1/70) |
Biolegend |
Cat#101218; RRID: AB_389327 |
| AF647TM anti-mouse CD29 (β1) (clone HA2/5) |
BD Biosciences |
Cat#562153; RRID: AB_10896298 |
| AF647TM anti-mouse Fcer1g (clone E12) |
Santa Cruz |
Cat#Sc-390222; RRID: AB_2938815 |
| AF700TM anti-mouse CD11c (clone N418) |
eBioscience |
Cat#56-0114-82; RRID: AB_493992 |
| Anti-mouse DUSP2 |
Thermo Fisher |
Cat#PA5-26093; RRID: AB_2543593 |
| Biotin anti-mouse B220 (clone RA3-6B2) |
BD Biosciences |
Cat#553086; RRID: AB_394616 |
| Biotin anti-mouse CD11c (clone HL3) |
BD Biosciences |
Cat#553800; RRID: AB_395059 |
| Biotin anti-mouse NK1.1 (clone PK136) |
BD Biosciences |
Cat#553163; RRID: AB_394675 |
| Biotin anti-mouse CD4 (clone GK1.5) |
BD Biosciences |
Cat#553728; RRID: AB_395012 |
| Biotin anti-mouse CD8α (clone 53-6.7) |
BD Biosciences |
Cat#553029; RRID: AB_394567 |
| Biotin anti-mouse CD11b (clone M1/70) |
BD Biosciences |
Cat#553309; RRID: AB_394773 |
| Biotin anti-mouse CD25 (clone 7D4) |
BD Biosciences |
Cat#553070; RRID: AB_394602 |
| Biotin anti-mouse TER-119 (clone TER-119) |
BD Biosciences |
Cat#553672; RRID: AB_394985 |
| Biotin anti-mouse CD44 (clone IM7) |
Biolegend |
Cat#103003; RRID: 312954 |
| Biotin anti-mouse CD49d (a4) (clone R1-2) |
Biolegend |
Cat#103603; RRID: AB_313034 |
| Guinea pig anti-mouse insulin |
Invitrogen |
Cat#PA1-26938; RRID: AB_794668 |
| Rabbit anti-mouse CD3 |
Abcam |
Cat#ab5690; RRID: AB_305055 |
| AF488TM donkey anti-guinea pig |
Jackson Immunoresearch |
Cat#706-545-148; RRID: AB_2340472 |
| CyTM3 donkey anti-rat |
Jackson Immunoresearch |
Cat#712-165-153; RRID: AB_2340667 |
| AF647TM donkey anti-rabbit |
Invitrogen |
Cat#A31573; RRID: AB_2536183 |
|
Bacterial and virus strains
|
| Type 1 Lang (T1L) |
Dermody Lab (University of Pittsburgh) |
N/A |
| T3SA+
|
Dermody Lab (University of Pittsburgh) |
N/A |
| MNV CW3 |
Randall Lab (University of Chicago) |
N/A |
|
Biological samples
|
| Bovine serum albumin (BSA) |
Thermo FIsher |
Cat#BP1600 |
| Normal donkey serum (NDS) |
Jackson ImmunoResearch |
Cat#017-000-121 |
| Newborn calf serum (NCS) |
GeminiBio |
Cat#100-504 |
| Penicillin-streptomycin solution |
Corning |
Cat#30001CI |
| Collagenase, Type IV |
Thermo Fisher |
Cat#17104019 |
| Collagenase, Type D |
Millipore Sigma |
Cat#11088866001 |
| Collagenase, Type VIII |
Millipore Sigma |
Cat#C2139 |
| Albumin from chicken egg white |
Millipore Sigma |
Cat#A5378 |
| Ovalbumin EndoFitTM
|
InvivoGen |
Cat#vac-pova |
|
Chemicals, peptides, and recombinant proteins
|
| TRIzolTM Reagent |
Thermo Fisher |
Cat#15596018 |
| DNasel |
Millipore Sigma |
Cat#10104159001 |
| Betaine (5M) |
Millipore Sigma |
Cat#B0300 |
| Histopaque-1077 |
Millipore Sigma |
Cat#10770 |
| RNAlater |
Millipore Sigma |
Cat#R0901 |
| Omeprazole |
Millipore Sigma |
Cat#O104 |
| Tamoxifen |
Millipore Sigma |
Cat#T5648 |
| Diphtheria toxin |
Millipore Sigma |
Cat#D0564 |
| Streptozotocin (STZ) |
Caymen Chemicals |
Cat#13104 |
| Low melt agarose |
Goldbio |
Cat#A20405 |
| Clear mounting media |
Thermo Fisher |
Cat#OB010020 |
| 2-mercaptoethanol |
Sigma Aldrich |
Cat#M7522 |
| RPMI 1640, powder |
Thermo Fisher |
Cat#31800105 |
| Percoll |
GE Healthcare |
Cat#17089109 |
| HEPES Buffer |
Corning |
Cat#25060CI |
| HIP2.5 peptide (LQTLALWSRMD) |
Lifetein Technologies |
N/A, custom order |
| LIVE/DEADTM Fixable Near-IR |
Thermo Fisher |
Cat#L10119
|
| LIVE/DEADTM Fixable Aqua |
Thermo Fisher |
Cat#L34965
|
| Zombie NIRTM
|
Biolegend |
Cat#423105 |
| CellTraceTM Violet |
Thermo Fisher |
Cat#C34557
|
| CellTraceTM CFSE |
Thermo Fisher |
Cat#C34554
|
| Trypan blue solution, 0.4% |
Thermo Fisher |
Cat#15250061 |
| Buffer TCL |
Qiagen |
Cat#1031576 |
|
Critical commercial assays
|
| Fixation/permeabilization Concentrate |
Thermo Fisher |
Cat#00-5123-43 |
| Fixation/permeabilization Diluent |
Thermo Fisher |
Cat#00-5223-56 |
| Cytofix/cytopermTM Fixation and Permeabilization Solution |
BD Biosciences |
Cat#554722 |
| Permeabilization Buffer (10X) |
Thermo Fisher |
Cat#00-8333-56 |
| GolgiPlugTM Protein Transport Inhibitor |
BD Biosciences |
Cat#555029 |
| eBioscienceTM Cell Stimulation Cocktail |
Thermo Fisher |
Cat#00-4975-93 |
| SuperscriptTM III One-Step RT-PCR System with PlatinumTM
Taq High Fidelity DNA Polymerase |
Thermo Fisher |
Cat#12574035 |
| Maxima H Minus Reverse Transcriptase (200 U/μL) |
Thermo Fisher |
Cat#EP0753 |
| RNasinTM Plus RNase Inhibitor |
Promega |
Cat#N2611 |
| HiFi HS Ready Mix 2X |
KAPA Biosystems |
Cat#K2601K |
| RNAClean XP beads |
Beckman Coulter |
Cat#A63987 |
| Ultra-Sensitive Mouse Insulin ELISA kit |
Crystal Chem |
Cat#90080 |
| Mouse IL-15 DuoSet ELISA |
R&D Systems |
Cat#DY447-05 |
| AldefluorTM Kit |
Stem Cell Technologies |
Cat#01700 |
| Anti-biotin Microbeads |
Miltenyi Biotec |
Cat#130090485 |
|
Deposited data
|
| Bulk RNA-seq |
This study |
GEO: GSE209701
|
| scRNA-seq |
This study |
GEO: GSE209702
|
|
Experimental models: Organisms/strains
|
| Mouse: C57BL/6J (B6) |
Jackson Laboratory |
MGI:3028467 |
| Mouse: B6.Cg-Tg(TcraTcrb)425Cbn/J (OT-II) |
Mucida Lab (Rockefeller University) |
MGI:2174541 |
| Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I) |
Jackson Laboratory |
MGI:2174540 |
| Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) |
Jackson Laboratory |
MGI:2164701 |
| Mouse: C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ (RIPmOVA) |
Jackson Laboratory |
MGI:3522666 |
| Mouse: B6.Cg-Tg(Itgax-Venus)1Mnz/J (CD11c-YFP) |
Nussenzweig Lab (Rockefeller University) |
MGI:3839312 |
| Mouse: B6.129S6(Cg)-Ptf1atm2(cre/ESR1)Cvw/J (Ptf1aCreERT2) |
Jackson Laboratory |
MGI:6719359 |
| Mouse: B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J (VillinCreERT2) |
Jackson Laboratory |
MGI:6278020 |
| Mouse: B6.Cg-Tg(Vil1-cre)1000Gum/J (Villincre) |
Jackson Laboratory |
MGI:5474782 |
| Mouse: B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J (Albcre) |
Jackson Laboratory |
MGI:2164673 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (TdTomato) |
Jackson Laboratory |
MGI:3813512 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J (ZsGreen) |
Jackson Laboratory |
MGI:3813510 |
| Mouse: C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J (iDTR) |
Jackson Laboratory |
MGI:3772860 |
| Mouse: NOD/ShiLtJ (NOD) |
Jackson Laboratory |
MGI:2162056 |
| Mouse: NOD.NON-Thy1a/1LtJ (NOD Thy1.1) |
Jackson Laboratory |
MGI:2386298 |
| Mouse: NOD.129S7(B6)-Rag1tm1Mom/J (NOD Rag1−/−) |
Jackson Laboratory |
MGI:2165365 |
| Mouse: NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ (NOD BDC2.5) |
Jackson Laboratory |
MGI:2183909 |
| Mouse: NOD.Cg-Tg(TcraTcrbNY8.3)1 Pesa/DvsJ (NOD NY8.3) |
Jackson Laboratory |
MGI:3603341 |
| Mouse: NOD.G9C8 |
Chervonsky Lab (University of Chicago) |
N/A |
|
Oligonucleotides
|
|
Aldh1a2_Fwd |
Integrated DNA Technologies |
ATGGATGCGTCTGAAAGAGG |
|
Aldh1a2_Rev |
Integrated DNA Technologies |
TGACTCCCTGCAAATCGATG |
|
Ly6a_Fwd |
Integrated DNA Technologies |
GGATGGACACTTCTCACACTAC |
|
Ly6a_Rev |
Integrated DNA Technologies |
GCAGGTAATTGATGGGCAAG |
|
Anxa2_Fwd |
Integrated DNA Technologies |
GTCTACTGTCCACGAAATCCTG |
|
Anxa2_Rev |
Integrated DNA Technologies |
ACTCCTTTGGTCTTGACTGC |
|
Ccl6_Fwd |
Integrated DNA Technologies |
TCTTTATCCTTGTGGCTGTCC |
|
Ccl6_Rev |
Integrated DNA Technologies |
ATGGGATCTGTGTGGCATAAG |
|
Ccl5_Fwd |
Integrated DNA Technologies |
GGGTACCATGAAGATCTCTGC |
|
Ccl5_Rev |
Integrated DNA Technologies |
TCTAGGGAGAGGTAGGCAAAG |
|
Apol7c_Fwd |
Integrated DNA Technologies |
TGTATGCACAGATGGTCACTG |
|
Apol7c_Rev |
Integrated DNA Technologies |
CAGGCAGAGGACTTAATAGGTG |
|
36B4_Fwd |
Integrated DNA Technologies |
GCCGTGATGCCCAGGGAAGAC |
|
36B4_Rev |
Integrated DNA Technologies |
CATCTGCTTGGAGCCCACGTT |
|
Isg15_Fwd |
Integrated DNA Technologies |
GGTGTCCGTGACTAACTCCAT |
|
Isg15_Rev |
Integrated DNA Technologies |
TGGAAAGGGTAAGACCGTCCT |
|
Stat1_Fwd |
Integrated DNA Technologies |
AGGGGCCATCACATTCACAT |
|
Stat1_Rev |
Integrated DNA Technologies |
AGATACTTCAGGGGATTCTC |
|
Mx1_Fwd |
Integrated DNA Technologies |
GACCATAGGGGTCTTGACCAA |
|
Mx1_Rev |
Integrated DNA Technologies |
AGACTTGCTCTTTCTGAAAAGCC |
|
Il15ra_Fwd |
Integrated DNA Technologies |
CTACATCGGTCCTCTTGGTTG |
|
Il15ra_Rev |
Integrated DNA Technologies |
GCCCTCACAGTCATTGGTAC |
|
Ifit3_Fwd |
Integrated DNA Technologies |
CTGAAGGGGAGCGATTGATT |
|
Ifit3_Rev |
Integrated DNA Technologies |
AACGGCACATGACCAAAGAGTAGA |
|
Fcer1g_Fwd |
Integrated DNA Technologies |
TATAGCCAGCCGTGAGAAAGC |
|
Fcer1g_Rev |
Integrated DNA Technologies |
AGCCAAGCACGTCTGTTCTG |
|
Il12b_Fwd |
Integrated DNA Technologies |
ACTCCCCATTCCTACTTCTCC |
|
Il12b_Rev |
Integrated DNA Technologies |
CATTCCCGCCTTTGCATTG |
|
S100a10_Fwd |
Integrated DNA Technologies |
GCACTAGCCTCATCGTGG |
|
S100a10_Rev |
Integrated DNA Technologies |
CTGCAAACCTGTGAAACGTAAG |
|
Hic1_Fwd |
Integrated DNA Technologies |
GCATCTTGCTCCCGTCTTCC |
|
Hic1_Rev |
Integrated DNA Technologies |
CCCAGCACACTCTCCCGATTTA |
|
Asb2_Fwd |
Integrated DNA Technologies |
TGCAGAGAACACCTGGATTG |
|
Asb2_Rev |
Integrated DNA Technologies |
GTATCGCACCAATATCCTCACC |
|
Egr1_Fwd |
Integrated DNA Technologies |
TGAGCACCTGACCACAGAGTC |
|
Egr1_Rev |
Integrated DNA Technologies |
TGAAAAGGGGTTCAGGCCAC |
|
Ifitm6_Fwd |
Integrated DNA Technologies |
ACATCTACTCGGTGAAGTCCAGG |
|
Ifitm6_Rev |
Integrated DNA Technologies |
GGCGGTTGAAGCATGGGATT |
|
Ier2_Fwd |
Integrated DNA Technologies |
AAGAGGAAGTGCTGCGAGTC |
|
Ier2_Rev |
Integrated DNA Technologies |
TAGACGGGCCTTCTTGCTTG |
|
Arf4_Fwd |
Integrated DNA Technologies |
GCTGTGCTGCAGAAAATGCTTC |
|
Arf4_Rev |
Integrated DNA Technologies |
AGCACAAGTGGCTTGGACAT |
|
Ppp1r15a_Fwd |
Integrated DNA Technologies |
GCCTGTGAAACATTGCGTCC |
|
Ppp1r15a_Rev |
Integrated DNA Technologies |
CCATGTGTCTGGGCGGC |
|
Ccr7_Fwd |
Integrated DNA Technologies |
AAAGCACAGCCTTCCTGTGT |
|
Ccr7_Rev |
Integrated DNA Technologies |
AGTCCACCGTGGTATTCTCG |
|
Id3_Fwd |
Integrated DNA Technologies |
AGCTTTTGCCACTGACCC |
|
Id3_Rev |
Integrated DNA Technologies |
AGATCGAAGCTCATCCATGC |
|
Cd34_Fwd |
Integrated DNA Technologies |
CTGACTTGAGAAAGCTGGGGAT |
|
Cd34_Rev |
Integrated DNA Technologies |
AGCCATCAAGGTTCCAGCTC |
|
Emp3_Fwd |
Integrated DNA Technologies |
CCTGTCCTTCATCCTCTTCATG |
|
Emp3_Rev |
Integrated DNA Technologies |
GGTGTGGATGGCATAGATGAG |
|
Apol10b_Fwd |
Integrated DNA Technologies |
GGAGCCTGATAACTGAAGATGG |
|
Apol10b_Rev |
Integrated DNA Technologies |
CTCCTGTGCTAAACTCTCCTTC |
|
Dusp2_Fwd |
Integrated DNA Technologies |
GCGGTTTCAAAAGCTTCCAG |
|
Dusp2_Rev |
Integrated DNA Technologies |
TAGGGCAAGATTTCCACAGG |
|
S100a4_Fwd |
Integrated DNA Technologies |
TGAACAAGACAGAGCTCAAGG |
|
S100a4_Rev |
Integrated DNA Technologies |
GAAGACACAGTACTCCTGGAAG |
|
S100a6_Fwd |
Integrated DNA Technologies |
ACAAGTACTCTGGCAAGGAAG |
|
S100a6_Rev |
Integrated DNA Technologies |
GATCCTTGTTACGGTCCAGATC |
|
Gpr183_Fwd |
Integrated DNA Technologies |
GCCTATCACAGTCATTCTCCTG |
|
Gpr183_Rev |
Integrated DNA Technologies |
CACAGGATGAACACGACAATG |
|
Fth1_Fwd |
Integrated DNA Technologies |
TCAACCGCCAGATCAACC |
|
Fth1_Rev |
Integrated DNA Technologies |
TCAGTTTCTCGGCATGCTC |
|
Ftl1_Fwd |
Integrated DNA Technologies |
CAGCCATGACCTCTCAGATTC |
|
Ftl1_Rev |
Integrated DNA Technologies |
CCACGTCATCCCGATCAAAA |
|
Ebi3_Fwd |
Integrated DNA Technologies |
CAAGGAACAGAGCCACAGAG |
|
Ebi3_Rev |
Integrated DNA Technologies |
GGGATACCGAGAAGCATGG |
|
Cd40_Fwd |
Integrated DNA Technologies |
CGGTCCATCTAGGGCAGTGT |
|
Cd40_Rev |
Integrated DNA Technologies |
CTGGCTGGCACAAATCACAG |
|
Ifnar1_Fwd |
Integrated DNA Technologies |
TCTCTGTCATGGTCCTTTATGC |
|
Ifnar1_Rev |
Integrated DNA Technologies |
CTCAGCCGTCAGAAGTACAAG |
|
Ifnar2_Fwd |
Integrated DNA Technologies |
GTGACAGATAAGTGGTTGGAGG |
|
Ifnar2_Rev |
Integrated DNA Technologies |
ACGATCTCAAATTCTGGCGG |
|
Il15_Fwd |
Integrated DNA Technologies |
CAT ATGGAATCCAACTGGAT AGATGTAAGATA |
|
Il15_Rev |
Integrated DNA Technologies |
CATATGCTCGAGGGACGTGTTGATGAACAT |
|
T1L_Fwd |
Thermo Fisher |
CGCTTTTGAAGGTCGTGTATCA |
|
T1L_Rev |
Thermo Fisher |
CTGGCTGTGCTGAGATTGTTTT |
|
T1L_probe |
Thermo Fisher |
FAM-AGCGCGCAAGAGGGATGGGA-BNFQ |
|
Software and algorithms
|
| GraphPad Prism (v9.3.1) |
GraphPad |
www.graphpad.com
|
| FlowJo (v10.8.1) |
Tree Star |
https://www.flowjo.com/
|
| Adobe Illustrator (v25.4.1) |
Adobe |
www.adobe.com/products/illustrator
|
| R (v4.0.5) |
The R Foundation |
https://www.r-project.org/
|
| Kallisto |
Bray et al. |
https://pachterlab.github.io/kallisto/about
|
| DE-Seq2 (v1.30.1) |
Love et al. |
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
| CellRanger (v3.0) |
10X Genomics |
https://www.10xgenomics.com/support
|
| Seurat (v4.1.0) |
Hao et al. |
https://github.com/satijalab/seurat
|
|
Devices
|
| BD LSRII |
BD Biosciences |
N/A |
| 4-laser Aurora spectral flow cytometer |
Cytek Bioscience |
N/A |
| Vibrating blade microtome |
Leica |
N/A |
| EVOSTM FL fluorescent microscope |
Invitrogen |
N/A |
| SP5 confocal microscope |
Leica |
N/A |
