TABLE 2.
Sequence changes of 25 BLV LTR mutants made by site-directed mutagenesis
| Namea | Site of mutationb | Original sequence | Mutated sequence |
|---|---|---|---|
| d-CRE1 | −157 to −153 | AGACG | Deleted |
| d-CRE2 | −131 to −129 | GAC | Deleted |
| d-CRE3 | −57 to −54 | TGAC | Deleted |
| s-CRE3 | −56 | G | T |
| d-CRE 1/2 | −157 to −153 | AGACG | Deleted |
| −131 to −129 | GAC | Deleted | |
| d-CRE 1/3 | −157 to −153 | AGACG | Deleted |
| −57 to −54 | TGAC | Deleted | |
| dCRE 1/s-GRE | −157 to −153 | AGACG | Deleted |
| −71 to −60 | ACA---TG-CT | TAT---AC--TA | |
| dCRE2/s-GRE | −131 to −129 | GAC | Deleted |
| −71 to −60 | ACA---TG--CT | TAT---AC--TA | |
| d-CRE2/3 | −131 to −129 | GAC | Deleted |
| −57 to −54 | TGAC | Deleted | |
| d-CRE3/a-GRE | −57 to −54 | TGAC | Deleted |
| −71 to −72 | Wild type | TATCC added | |
| d-CRE1/2/3 | −157 to −153 | AGACG | Deleted |
| −131 to −129 | GAC | Deleted | |
| −57 to −54 | TGAC | Deleted | |
| d-CRE1/2/s-GRE | −157 to −153 | AGACG | Deleted |
| −131 to −129 | GAC | Deleted | |
| −71 to −60 | ACA---TG--CT | TAT---AC--TA | |
| d-CRE2/3/a-GRE | −131 to −129 | GAC | Deleted |
| −57 to −54 | TGAC | Deleted | |
| −71 to −72 | Wild type | TATCC added | |
| d-CRE1/2/3/a-GRE | −157 to −153 | AGACG | Deleted |
| −131 to −129 | GAC | Deleted | |
| −57 to −54 | TGAC | Deleted | |
| −71 to −72 | Wild type | TATCC added | |
| s-GRE | −71 to −60 | ACA---TG--CT | TAT---AC--TA |
| s-167 | −167 | A | G |
| s-AP4-1 | −151 | G | T |
| s-142,3 | −142 to −143 | AG | CT |
| s-140 | −140 | A | G |
| s-AP4-2 | −124 | G | T |
| s-116 | −116 | C | A |
| s-107,8 | −107 to −108 | CC | AT |
| s-99 | −99 | C | T |
| s-83,4 | −83 to −84 | TC | CT |
| s,a-TATA | −41 to −40 | TA | AT |
| −37 to −38 | Wild type | T added |
a
Abbreviations: a, addition; d, deletion; s, substitution; AP4, activating protein 4.
b
Positions of sequence changes are indicated by the numbers which represent the distance (in base pairs) relative to the RNA start site (+1).