FIG. 1.
Subcellular localization of HIV-1 Vpr in HeLa cells. Monolayer cultures were transfected with vectors that expressed wild-type Vpr (A, B, E, and F) or the VprA30P mutant (C, D, G, and H) as either Myc-tagged proteins (A to D) or fusions to MBP (E to H). Expressed proteins were detected by indirect immunofluorescence with either the Myc-specific monoclonal antibody (A and C) or an MBP-specific antiserum (E and G), TXRD-conjugated secondary antibodies, and epifluorescence. The corresponding phase-contrast analyses are also shown (B, D, F, and H).