Figure 2.

UBAP2L interacts with Nups and NPC assembly factors. (A) HeLa cells lysates expressing GFP alone or 3XGFP-Nup85 for 27 h were immunoprecipitated using agarose GFP-Trap A beads (GFP-IP), analyzed by western blot, and signal intensities were quantified (shown a mean value, **P < 0.01, ***P < 0.001, unpaired two-tailed t test; n = 3 independent experiments). Molecular weight markers are indicated in kilodalton (kDa). Please note that in all shown experiments, a specific band corresponding to Nup153 and recognized by both Nup153 and mAb414 antibodies displayed an atypical migration pattern of around 250 kDa size, probably due to usage of Tris-acetate gradient gels (Materials and methods section). (B) HeLa cells lysates were immunoprecipitated using UBAP2L antibody or unspecific rabbit IgG, analyzed by western blot, and signal intensities were quantified (shown a mean value, **P < 0.01, ***P < 0.001, unpaired two-tailed t test; n = 3 independent experiments). The arrow indicates the band corresponding to the IgG heavy chain (HC). (C) Lysates of HeLa cells expressing GFP alone or GFP-FXR1 for 27 h were immunoprecipitated using agarose GFP-Trap A beads (GFP-IP), analyzed by western blot, and signal intensities were quantified (shown a mean value, *P < 0.05, **P < 0.01, unpaired two-tailed t test; n = 3 independent experiments). Source data are available for this figure: SourceData F2.