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Adding back Nrf1 in Nrf1-deficient cells rescues suppressed expression of ALP genes upon proteasome inhibition. SH-SY5Y-Nrf1KD and HT22-Nrf1KO cells were infected with Nrf1(p120). Both SH-SY5Y-Nrf1KD, p120 rescue and HT22-Nrf1KO, p120 rescue cells were treated with 200 nM BTZ for 6 h and then analyzed by qRT-PCR to measure the expression levels of indicated genes, and mRNA levels of 18S rRNA levels was used for normalization.
