Fig. 3. Respiratory tract CD8 T_RM protect against viral propagation following transmission through IFNγ.

(a) Schematic of experimental setup where immunized contact mice were cohoused with a Sendai-Luc infected index mouse. (b) Bioluminescence curves of contact mice immunized with PR8 WT i.n. (n=13), PR8 SenNP i.p. (n=16), or PR8-SenNP i.n. (n=15). (c) AUC of bioluminescence in immunized contact mice that become infected following co-housing with an infected index mouse. (d) Probability of infection for immunized contact mice, calculated as the proportion of contact mice that became infected. (e) Schematic of experiment to investigate protection from transmission in immunized WT, Prf^−/− Ifng^−/−, and Ifngr^−/− contact mice and WT mice treated with anti-FasL antibody. (f) Bioluminescence curves of immunized WT (n=16 for LAIV WT and LAIV-SenNP immunization), Prf^−/− (n=16), Ifng^−/−, (n=13), Ifngr^−/− (n=8), and WT+anti-FasL (n=14) contact mice after co-housing with an infected index mouse. (g) AUC of bioluminescence in contact mice that become infected following co-housing with an infected index mouse. (h) Probability of infection for immunized contact mice. Error bars represent 95% binomial confidence intervals (d, h). All data are combined from 2 independent replicates. For bioluminescence curves (b, f), solid dark lines represent means, solid pale lines represent individual mice, dashed grey line represents background bioluminescence, and dashed red line represents the limit of infection. Solid lines (c, d, g, h) and error bars (b, c, f, g) represent mean with 95% confidence interval. Statistical significance was determined using two-sided Mann Whitney test. * p<0.05, *** p<0.001, **** p<0.0001, ns: non-significant.