Figure 4.

Density of α-synuclein bound to a supported lipid bilayer measured with a Total Internal Reflection Fluorescence (TIRF) Microscopy. The dotted blue line shows a fit to the Adair eqaution⁴⁷ assuming one binding site, which corresponds to independent binding. The red, purple, and gray lines show fits to Adair equations assuming two, tree, and four coupled binding sites, respectively. N is the number of coupled binding sites. Reproduced from ref (43). Copyright 2021 American Chemical Society.