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. 2024 Apr 24;24:321. doi: 10.1186/s12870-024-05012-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Protein and protein interaction between OsNAR2.1 and OsPLDα1. A: Testing the interaction of OsNAR2.1 and OsPLDα1 using the DUAL pairwise interaction kit with ADE2 and HIS3 as reporter genes. Yeast strain NMY51 carried each pair of bait and prey plasmid, co-expression of OsNAR2.1 and OsNRT2.3a as a positive gene control for protein interactions (Yan et al., 2011; Liu et al., 2014), pBT3-C and pPR3-N are the control vectors with no cloned cDNA. Cells grown on selective SD/-Ade/-His/-Leu/-Trp or control SD/-Leu/-Trp medium. B: Bimolecular fluorescent complementary assays to detect the interaction between OsPLDα1 and OsNAR2.1. The ORFs of OsPLDα1 and OsNAR2.1 were constructed on the PSAT1-cEYFP-N1 vector and PSAT1-nEYFP-C1 vector, respectively. Fluorescence could only be detected when OsPLDα1-cEYFP was combined with nEYFP-OsNAR2.1. Bars, 10 μm