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. 1998 Jul;72(7):6034–6039. doi: 10.1128/jvi.72.7.6034-6039.1998

FIG. 2.

FIG. 2

FIG. 2

Competition of RAM23 and EBNA2 for their interaction with RBP-J. (A) GST-RAM23 coprecipitates 35S-labeled RBP-J but not 35S-labeled EBNA2. Added samples in each lane are shown above. 35S-labeled in vitro-translated RBP-J (lane 1) or EBNA2 (lane 2) was clearly distinguished by size. Samples in lanes 3 to 8 were coprecipitated as described previously (22). (B) GST-EBNA2 coprecipitates 35S-RBP-J but not 35S-RAM23. Added samples in each lane are shown above. Samples in lanes 3 to 6 were coprecipitated. (C) EMSA for ternary complex formation among EBNA2, RAM23, and RBP-J. EMSA was carried out as previously described (7) with 2 ng of 32P-labeled Epstein-Barr virus Cp promoter probe (16). Added samples other than DNA probe are listed above. Arrowheads indicate the sample added at the end. Sequential addition of both GST-EBNA2 and GST-RAM23 did not change the mobility of the supershifted band with either GST-RAM23 or GST-EBNA2 alone (lanes 7 and 8). Neither GST-RAM23 nor GST-EBNA2 formed a complex with the probe (data not shown). Note that the complex of RBP-J and GST-EBNA2 migrates slightly faster than that of RBP-J and GST-RAM23. S, supershifted band.

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