FIG. 4.

Suppression of myogenic differentiation of C2C12 cells by RAMIC and EBNA2 mutants. RAMIC (A), EBNA2 (B), and EBNA2 mutant proteins (C and D) are stained green and differentiated cells, judged by myoglobin expression, are stained red. Note that nuclei of transfected cells that underwent differentiation look yellow because of overlap with the red staining of myoglobin. Green nuclei indicate expression of RAMIC or EBNA2 or EBNA 2 mutants without differentiation into myotube. Differentiation of C2C12 cells is suppressed strongly with RAMIC (A) and EBNA2 (B). No or weak, if any, differentiation suppression was observed for EBNA2 WW323SS (C) and EBNA2 ΔHincII-StuI (D).