Table 1.
The primer sequences used in this study
| Target gene | Oligonucleotide sequence (5′ to 3′) | Annealing temperature (◦C) | Product size (bp) | Reference |
|---|---|---|---|---|
| bla TEM |
F: GAGTATTCAACATTTCCGTGTC R: TAATCAGTGAGGCACCTATCTC |
60 | 800 | 17 |
| bla SHV | F: CGCCTGTGTATTATCTCCCTGTTAGCC | 60 | 843 | 17 |
| R: TTGCCAGTGCTCGATCAGCG | ||||
| bla CTX−M−14 | F: TTATGCGCAGACGAGTGCGGTG | 55 | 120 | 19 |
| R: TCACCGCGATAAAGCACCTGCG | ||||
| bla CTX−M−15 |
F: GAGCCGACGTTAAACACCGCCA R: GCTGCACCGGTGGTATTGCCTT |
58 | 156 | 19 |
| bla CIT |
F: TGGCCAGAACTGACAGGCAAA R: TTTCTCCTGAACGTGGCTGGC |
57 | 462 | 17 |
| bla MOX |
F: GCTGCTCAAGGAGCACAGGAT R: CACATTGACATAGGTGTGGTGC |
57 | 520 | 17 |
| bla DHA | F: AACTTTCACAGGTGTGCTGGGT | 57 | 405 | 17 |
| R: CCGTACGCATACTGGCTTTGC | ||||
| bla EBC |
F: TCGGTAAAGCCGATGTTGCGG R: CTTCCACTGCGGCTGCCAGTT |
57 |
302 Variable |
17 |
| ERIC |
F: ATGTAAGCTCCTGGGGATTCAC R: AAGTAAGTGACTGGGGTGAGCG |
51 | 11 |