FIG. 3.

Determination of the origins of the two forms of HCV core proteins, p21 and p23 by Western blot analysis. Plasmids pT7THC154, pT7THC173, and pT7THC191 were transiently expressed in an in vitro translation system (lanes 1, 3, and 5, respectively) and were also transfected into RK13 cells, which were then infected with LO-T7-1 RVV and harvested 12 h after infection (lanes 2, 4, and 6, respectively). Plasmid pT7THCN1134 was also transfected into RK13 cells, which were then infected with LO-T7-1 RVV and harvested 12 h after infection (lanes 7). RK13 cells infected with both LO-T7-1 RVV and LO-R6J20 RVV were then harvested 3 h after infection (lane 8). The same sample was loaded in lanes 6 and 9.