Table 1.
Primer name | Primer Sequences 3’ to 5’ | Annealing temperature(°C) | Purpose | Product length |
---|---|---|---|---|
P1 |
F-TCAGACCTCTTCCCCCTCATCT R-GAGCTCACCTGTGGATGCAG |
58 | Polymorphism | 600bp |
P2 |
F-AAAGAGGGGTTGTGAGCTTG R-CACCTCGATGTCCTCTGATG |
55 | Polymorphism | 464bp |
P3 |
F-GGTGGCAGGGAACTGATTTA R-GACACAGATTTGCGTCCACT |
55 | Polymorphism | 826bp |
P4 |
F-GCTGTTGTCCTCATGAACCT R-TGAGATTATGTCGCTTCGCC |
55 | Polymorphism | 764bp |
P5 |
F-TCCAGCTGGGAAGAGCAA R-AGGAAGTCACCGGTTCCT |
55 | Polymorphism | 600bp |
P6 |
F-TCTCCTCCAGCTCCCCA R-AGAAGGGCTGAGAAGTCCAT |
51 | Polymorphism | 620bp |