FIG. 2.

T-cell responses of PBMC 6 weeks after experimental WHV infection and of rWHcAg-specific T-cell lines established at 6, 12, 18, and 24 weeks postinfection to 1 μg of rWHcAg or WHcAg peptides per ml were analyzed by [2-³H]adenine incorporation. PBMC (5 × 10⁴) were obtained from woodchucks NW7029, NW7030, NW7031, and NW7032 6 weeks after infection. T cells (5 × 10⁴) were derived from polyclonal T-cell lines established at weeks 6, 12, 18, and 24 by continuous stimulation with rWHcAg. Results are presented as mean SI of triplicate determinations. The mean cpm for the controls was 7,043 ± 3,368.