TABLE 2.
Peptide | PBMC response (mean SI) of woodchucka:
|
|||
---|---|---|---|---|
NW7029 | NW7030 | NW7031 | NW7032 | |
Panel A | ||||
1–20 | 7.8 | 8.3 | 2.8 | 8.7 |
15–34 | 1.6 | 1.6 | 1.5 | 1.5 |
28–47 | 7.3 | 2.1 | 1.9 | 1.4 |
38–57 | 6.7 | 7.2 | 2.5 | 2.0 |
50–69 | 2.8 | 2.5 | 1.3 | 2.6 |
61–80 | 1.4 | 1.4 | 1.8 | 2.3 |
70–89 | 6.4 | 6.9 | 2.7 | 1.3 |
82–101 | 1.9 | 1.3 | 1.2 | 2.1 |
90–109 | 5.9 | 6.1 | 1.4 | 2.3 |
100–119 | 8.1 | 8.4 | 8.8 | 7.5 |
112–131 | 2.9 | 3.7 | 8.2 | 4.3 |
120–139 | 3.7 | 8.0 | 7.0 | 5.2 |
131–150 | 2.1 | 2.1 | 1.4 | 1.7 |
146–165 | 1.3 | 1.5 | 1.0 | 1.3 |
156–175 | 1.7 | 1.8 | 1.7 | 6.2 |
169–188 | 2.5 | 2.0 | 1.6 | 6.9 |
Panel B | ||||
97–110 | 8.2 | 9.1 | 8.6 | 8.3 |
100–113 | 7.6 | 8.7 | 7.9 | 8.0 |
111–124 | 7.1 | 6.2 | 6.0 | 5.9 |
120–131 | 3.9 | 5.1 | 4.8 | 4.7 |
124–137 | 2.4 | 1.9 | 2.7 | 1.9 |
129–140 | 1.7 | 1.4 | 1.8 | 1.4 |
PBMC responses 6 weeks after experimental WHV infection to 1 μg of WHcAg peptides per ml were analyzed by [2-3H]adenine incorporation. PBMC (5 × 104) were obtained from woodchucks NW7029, NW7030, NW7031, and NW7032. Results are presented as mean SI of triplicate determinations. SI values of ≥3.1 are indicated in bold type. The mean cpm for the controls was 2,863 ± 1,397.