FIG. 5.

Reactivity of anti-gL antibodies with gL168 or with full-length gL. CHO-K1 cells were transfected with pMN115 (gL168) (lanes 1, 3, 5, 7, and 9) or pCMV3gL (gL) (lanes 2, 4, 6, 8, and 10). Cell extracts were prepared and resolved on a 12% polyacrylamide denaturing gel. After Western blotting, separate strips of the membrane were probed with antibodies L1, L2, L3, 8H4, or αUL1-1, as indicated below the gel. Secondary antibodies were then added, and ECL was used to visualize the bands.