FIG. 2.

Expression of Apj and CCR9 in PBMC. mRNA was isolated from PBMC activated with PHA (right-hand lanes) or with PHA plus IL-2 (left-hand lanes) and reverse transcribed to cDNA. PCR was performed with primers bounding an approximately 1,100-bp fragment of the Apj or CCR9 gene, or a 200-bp fragment of the β-globin gene control. In the lanes labeled RT−, the PCR was performed with the same reaction mixes used in the other two lanes, but prepared without reverse transcriptase.