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Apj supports the entry of replication-competent viruses. Replication-competent ELI (A) and 89.6 (B) HIV-1 isolates were incubated with Cf2Th cells transfected with CD4 and either a control plasmid or plasmids encoding Apj or CXCR4, in the presence or absence of 50 μM azidothymidine (AZT). The p24 values from 4 days after infection are given in picograms per milliliter, with a 1:5 dilution of cell supernatant.
