Extended Data Fig. 7. NAD⁺ treatment causes downregulation of genes enriched for functions related to immune responses and extracellular matrix remodelling exclusively in Kdm8 mutant hearts.

a, Percentage of differentially expressed genes (DEGs) in 2-month-old Kdm8^fl/fl;Myh6-Cre mutant hearts at disease onset (total = 458 genes) and that are persistently dysregulated in 4-month old mutant hearts at baseline, and in response to NAD⁺ treatment. Left bars represent the percentage of DEGs that were upregulated or downregulated at 2 months. Middle and right bars represent the percentage of those DEGs that are persistently dysregulated at 4 months. b, Gene ontology (GO) terms (cellular component) and KEGG pathways enriched amongst genes downregulated in Kdm8^fl/fl control and Kdm8^fl/fl;Myh6-Cre mutant mice at baseline and in response to NAD⁺ treatment. c, GO term (cellular component) and KEGG pathway (cellular component) enrichment amongst upregulated genes. Data was analyzed using g:Profiler. d, GO term (biological process) enrichment amongst upregulated genes. Data was analyzed using g:Profiler