Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 13;2(2):174–191. doi: 10.1038/s44161-023-00214-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a, Heatmap clustering of genes differentially expressed in ventricles of 2-month-old Kdm8^fl/fl;Myh6-Cre mutant vs Kdm8^fl/fl control mice. Enriched GO terms and representative genes are shown. b, GSEA including all expressed genes. P values were obtained from GSEA. NES, normalized enrichment score. c, Western blot of electron transport chain complexes I (Ndufb8) and IV (Cytochrome C) on 2-month-old ventricles. d, Protein relative to histone H3 in c. Data are mean ± s.e.m. Two-tailed Student’s t-test. n = 4 control and 5 mutant hearts. e, Transmission electron micrographs of the left ventricle of 2-month-old controls and mutants. Arrowheads indicate mitochondria. Scale bar, 1 μm. f, Mitochondria per 10 μm², and percentage area occupied by mitochondria per micrograph. Data are mean ± s.d. Two-tailed Student’s t-test. n = 5 mice per group. g, Heatmap of H3K36me2 (red) occupancy (normalized counts) in 2-month-old control and mutant hearts 1 kb upstream and downstream of the transcription start site (TSS). h, H3K36me2 enrichment as reads per kilobase per million mapped reads (RPKM) 1 kb upstream and downstream of the TSS in 2-month-old control and mutant hearts. TES, transcription end site. Dots indicate P values. i, Peaks with increased or decreased H3K36me2 across promoters, introns, exons and intergenic regions in 2-month-old Kdm8 mutant hearts. j, H3K36me2 occupancy RPKM 3 kb upstream and downstream of the TSS of genes that were downregulated or upregulated in 2-month-old Kdm8 mutant hearts. k, H3K36me2 CUT&Tag and RNA-seq tracks of Nampt in 2-month-old control and mutant hearts. Units for CUT&Tag are RPKM and reads per million mapped reads (RPM) for RNA-seq. l, Boxplot showing the mean and range (minimum to maximum) log fold change expression of genes encoding proteins that localize to the mitochondria (sourced from MitoCarta3.0), and of all genes with increased H3K36me2 at promoters or gene bodies. Unpaired two-tailed Student’s t-test. The number of genes in each compared category is indicated below each bar.

Source data