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. 2023 Feb 13;2(2):174–191. doi: 10.1038/s44161-023-00214-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Percentage of genotypes of male offspring from control Kdm8^fl/fl females crossed with mutant Kdm8^fl/fl;Myh6-Cre males. Data was analyzed by two-tailed chi-squared test. b, qPCR of Kdm8 normalized to Rpl13a in whole ventricles of 2-month-old control, heterozygous (Kdm8^fl+;Myh6-Cre), and mutant mice. Error bars denote the mean + /- s.d. Data was analyzed by one-way ANOVA with Tuckey’s multiple comparison correction. n = 5 hearts per group. c, qPCR of Kdm8 in isolated cardiomyocytes (CMs) and in the non-cardiomyocyte cell fraction (non CMs) of 2-month-old control and mutant hearts. Error bars denote the mean + /- s.d. Data was analyzed by two-way ANOVA. n = 3 control and 5 mutant hearts. qPCR in b and c were repeated twice with similar results. d, Western blot of Kdm8 in whole ventricles at 6 months of age. e, Band intensity of Kdm8 protein normalized to histone H3 (H3) (shown in d) and analyzed by unpaired two-tailed Student’s t-test. n = 5 hearts per group. Error bars denote the mean + /- s.e.m. f, Histone demethylase (HDM) activity of Jumonji domain-containing proteins, shown as fluorescence units, on extracts of control and mutant hearts at 5 weeks of age. Error bars denote the mean + /- s.d. Data was analyzed by unpaired two-tailed Student’s t-test. n = 5 control and 4 mutant hearts. g, Western blot of H3K36me2 in whole ventricles at 2 months of age. h, Band intensity of H3K36me2 normalized to H3 (shown in g) and analyzed by two-tailed Student’s t-test. error bars denote the mean + /- s.e.m. n = 5 control and 5 mutant hearts.

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