Figure 3.

Subcellular localization of AtCpIscA. Arabidopsis protoplasts were transformed with plasmids that express the indicated gene constructs under control of the constitutive 35S cauliflower mosaic virus promoter. The nonfused GFP protein-coding sequence was used as a control. TP/IscA-GFP encodes the fusion of the CpIscA TP (1–55) to the N terminus of GFP, and full-length IscA GFP contains the full coding sequence of the IscA precursor fused to GFP. After 16 h of expression, cells were observed using a confocal laser-scanning microscope. Green fluorescence signals, chlorophyll red autofluorescence, and an overlay of green and red signals are shown.