Structural features of OsENTs. A, Multiple alignment of the predicted amino acid sequences of four OsENTs with that of AtENT1. Putative transmembrane helices are underlined. The asterisk highlights the amino acid in plant ENTs that corresponds to residue 33 of hENT1 and hENT2. The black dot marks the Gly residue at the 154 position of hENT1. B, Phylogenetic relationship of OsENT1, OsENT2, OsENT3, and AtENTs. Amino acid alignment of ENTs was performed using the ClustalW program at the DDBJ Web site. Bar = 0.1 amino acid substitutions per site.