Figure 3.

Growth analysis of yeast cells expressing OsENTs. Growth of yeast cells harboring expression plasmids containing OsENT1, OsENT2, OsENT3, OsENT4, or controls (BY4735; BY4735 plain recipient cells, ΔFUI1; ΔFUI1 plain recipient cells, pYES2; cells transformed with empty vector pYES2). A, Schematic representation of BY4735 strains used in B and C. B and E, Growth on synthetic minimal medium lacking uracil but containing Gal (20 g L⁻¹). C, Growth on synthetic minimal medium lacking uracil and adenine but containing adenosine (150 μm) and Gal. D, Schematic representation of ΔFUI1 strains used in E and F. F, Growth on synthetic minimal medium lacking uracil but containing fluorouridine (1 mm) and Gal. Cells were grown for 2 to 3 d at 30°C.