Table I.

Inhibition of OsENT2-mediated [³H]adenosine uptake in BY4735 ade2 yeast cells by nucleosides, nucleobases, and nucleotides

[³H]Adenosine uptake was examined at a substrate concentration of 2.0 μm for 2 min at pH 6.0. The concentrations of various additives were 20 μm. Mean ± se (n = 3).

Additive	[3H]Adenosine Uptake (% of Control)
None	100
Nucleosides:
Adenosine	23.8 ± 2.6
Cytidine	35.1 ± 4.0
Guanosine	20.4 ± 3.5
Uridine	13.3 ± 2.2
Inosine	16.6 ± 2.4
2′-Deoxyadenosine	37.5 ± 5.6
2′-Deoxycytidine	25.3 ± 3.8
2′-Deoxyguanosine	29.7 ± 3.3
2′-Deoxythymidine	6.7 ± 1.6
2′-Deoxyinosine	24.4 ± 3.2
Nucleobases:
Adenine	100.5 ± 0.7
Cytosine	94.6 ± 0.9
Guanine	98.8 ± 2.4
Thymine	89.7 ± 2.6
Uracil	85.7 ± 2.8
Hypoxanthine	79.7 ± 2.1
Nucleotides:
dATP	77.2 ± 2.8
ATP	73.5 ± 1.5
ADP	71.9 ± 0.3
AMP	72.3 ± 2.7
CTP	69.4 ± 2.8
GTP	62.9 ± 0.1
UTP	64.4 ± 1.0