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. 2023 Jan 30;2(2):112–125. doi: 10.1038/s44161-022-00208-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. Gating strategy used for fluorescent-activated cell sorting (FACS) to isolate plaque live CD45⁺ cells for 10X Genomics and sequencing. b. UMAP projection of all PBMC and plaque cells, depicting multiple leukocyte types (n = 33249). c. UMAP visualization of tissue distribution of PBMC and plaque cells. d. UMAP projection of protein expression of CD3, CD4, CD8 and CD14 on all PBMC and plaque cells. e. Patient contribution to UMAP of all PBMC and plaque cells. Red dots indicate cells that are retrieved from the abovementioned patient. f. Patient contribution to UMAP of PBMC and plaque T cells. Red dots indicate cells that are retrieved from the abovementioned patient.