Figure 5.

Fluorescent microscope images of cells transformed with egfp fusions comprised of truncated presequences. Images of a pre1-66-mptca1-egfp transformant (A) and a pre67-138-mptca1-egfp transformant (B). Light images, chlorophyll autofluorescence, GFP fluorescence, and merged images of chlorophyll and GFP fluorescence are presented, respectively, from the left column. Images were taken with the laser-scanning confocal microscope under conditions described in the text. Scale bars, 2 μm. Cells were transformed with construct c in Figure 1B (A), with construct d in Figure 1B (B), and grown in air-level CO₂.