Table II.
Identification of products formed after incubation of recombinant GA 2-oxidase (SoGA2ox3) from spinach with GA12 or GA53
| Substrate | Product | Mass Spectra of Productsa |
|---|---|---|
| m/z (% relative abundance) | ||
| 17,17-[2H2]GA12 | 17,17-[2H2]GA110 | M+ 450 (5), 435 (6), 418 (25), 390 (44), 375 (4), 360 (2), 328 (6), 318 (7), 300 (100), 285 (66), 274 (40), 260 (26), 259 (22), 241 (98), 225 (24), 201 (17), 197 (10), 145 (34) |
| 17,17-[2H2]GA53 | 17,17-[2H2]GA97 | M+ 538 (35), 523 (9), 506 (6), 479 (9), 448 (3), 389 (10), 373 (5), 329 (11), 299 (2), 239 (44), 210 (65), 209 (100), 195 (11), 179 (16), 147 (3), 119 (14) |
As the methyl ester trimethylsilyl ethers.