Table II.
Identification of products formed after incubation of recombinant GA 2-oxidase (SoGA2ox3) from spinach with GA12 or GA53
Substrate | Product | Mass Spectra of Productsa |
---|---|---|
m/z (% relative abundance) | ||
17,17-[2H2]GA12 | 17,17-[2H2]GA110 | M+ 450 (5), 435 (6), 418 (25), 390 (44), 375 (4), 360 (2), 328 (6), 318 (7), 300 (100), 285 (66), 274 (40), 260 (26), 259 (22), 241 (98), 225 (24), 201 (17), 197 (10), 145 (34) |
17,17-[2H2]GA53 | 17,17-[2H2]GA97 | M+ 538 (35), 523 (9), 506 (6), 479 (9), 448 (3), 389 (10), 373 (5), 329 (11), 299 (2), 239 (44), 210 (65), 209 (100), 195 (11), 179 (16), 147 (3), 119 (14) |
As the methyl ester trimethylsilyl ethers.