Table V.
GA content of N. sylvestris (Ns) and transgenic plants overexpressing SoGA2ox3 grown under various photoperiodic conditions
Plants were grown in SD for 80 d and then transferred to LD for the number of days indicated prior to harvest. GA content was determined by GC-MS-selected ion monitoring, using deuterated GAs as internal standards. nd, Not detectable.
| Sample | Photoperiodic Treatment | GA53 | GA19 | GA20 | GA1 | GA97 | GA110 |
|---|---|---|---|---|---|---|---|
| Ns | SD | 0.7a | 3.8 | 1.2 | 0.9 | 11.7 | nd |
| Ns/35S∷SoGA2ox3 | SD | 0.1 | 2.1 | 0.3 | 0.8 | 11.6 | 0.5 |
| Ns | 14 LD | 13.2 | 32.4 | 16.7 | 4.9 | 16.9 | nd |
| Ns/35S∷SoGA2ox3 | 14 LD | 4.4 | 4.7 | 0.8 | 0.9 | 40.3 | 7.2 |
| Ns | 20 LD | 32.3 | 54.3 | 27.0 | 5.2 | 14.1 | 1.5 |
| Ns/35S∷SoGA2ox3 | 20 LD | 6.3 | 7.5 | 1.8 | 1.0 | 85.3 | 9.1 |
| Ns/35S∷SoGA2ox3 | 30 LD | 9.7 | 9.4 | 2.3 | 1.1 | 94.9 | 10.2 |
Values are in ng g−1 dry weight.