FIG. 1.

Cell surface detection of gBpK⁻ mutant glycoprotein. Vero cells were transfected with expression plasmids encoding the wild-type gB protein (HCMV-BXX) (A) and the pK-deleted gB protein (HCMV-gBpK⁻) (B) or were mock transfected (C). After 30 h, unfixed transfected monolayers were incubated with a pool of monoclonal anti-gB antibody for 1 h at 4°C, washed with cold TBS, and incubated for 1 h at 4°C with a cy3 anti-mouse antibody. Monolayers were visualized with a Nikon microscope (model 211910) and photographed.