FIG. 4.

Comparative incorporation of glycoproteins B, C, and D into mutant virus envelopes. [³⁵S]methionine/cysteine-labeled sucrose-purified KOS (A), KgBpK⁻ (B), KCZ (C), KgBpK⁻gC⁻ (D), KgBpK⁻gC^R (E), and KgBpK^RgC⁻ (F) viruses were solubilized with detergent and immunoprecipitated with a pool of MAbs directed against gB (lanes 1), gC (lanes 2), or gD (lanes 3). The immune complexes were captured on protein A-Sepharose, resuspended in Laemmli buffer, and separated by SDS-PAGE. Asterisks indicate the positions of the glycoproteins designated on the left, when present.