FIG. 6.

Contribution of a viral glycoprotein(s) other than gB and gC to HS binding. Confluent monolayers of Vero cells in 12-well plates were inoculated with 100 PFU of each test virus per well for an adsorption period of 2 h at 4°C. The cells were then rinsed three times with complete medium in the absence or presence of 500 μg of heparin per ml, overlaid with medium containing methyl-cellulose, and shifted to 37°C to allow viral plaque formation. The cells were then fixed and stained with crystal violet, and plaques were counted. At each time point, the average number of plaques produced on heparin-washed monolayers was expressed as a percentage of the average number of plaques produced on complete medium-washed monolayers. Error bars indicate results determined for triplicate wells.