Figure 10.

Levels of GUS transcript and enzyme activity in different transgenic plants in response to Put and CHA. Detached Arabidopsis leaves were incubated for 12 h at room temperature in 10 mm Put or 50 mm CHA. A, Total RNA (10 μg per lane) isolated from transgenic plants with PBI121 (121, positive control), S, T, ST, and P5 was hybridized with DIG-labeled GUS-specific probe. B, The GUS activity in each transgenic plant was determined by GUS fluorometric assay. Bars represent mean ± se of three measurements.