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Schematic representation of different chimeric genes consisting of the wild type and mutated 5′ UTR flanking by the 35S promoter and GUS coding sequence. The binary vector PBI121 was inserted with the wild type (P5 without intron; P4 with intron) or mutated BJSAMDC2 5′ UTR (S, T, and ST). In the mutated 5′ UTR, mutagenized nucleotides in the uORFs are underlined and marked with an asterisk. The tiny and small uORFs are indicated as black and hatched boxes, respectively.
