Figure 6.

Phosphorylated H⁺-ATPase on plasma membrane. After an 8-d incubation in nutrient solution, Al-resistant soybean roots were treated in nutrient solution (pH 4.5) as follows. A, Duration of Al treatment was 2 h. B, Concentration of Al was 30 μm. After the treatment, the root apices (1 cm) were excised and used for preparation of plasma membrane H⁺-ATPase proteins. See details in “Materials and Methods.”