Figure 4.
Change in mRNA levels of P deprivation-responsive genes following exposure of wild-type cells and the lpb1 mutant to medium lacking P. A to C, The PHOX (A), PTB2 (B), and PSR1 (C) mRNA levels over a period of 24 h of P deprivation. Levels of the mRNA were quantified by qPCR, and the threshold cycle number or CT value was normalized to the amount of constitutively expressed CBLP transcript. The fold change was calculated relative to the amount of transcript at time 0 for wild-type cells.