Figure 5.

Quantification of ice recrystallization in the presence of apoplastic proteins from nonacclimated (NA) and cold-acclimated (CA) winter rye. Ice crystals that formed in nonacclimated and cold-acclimated apoplastic extracts containing (26%, w/w) Suc were measured after holding at −7°C for 3 to 53 min to allow recrystallization. The blank (B) was a solution of 26% Suc. Data were calculated as equivalent diameter and as aspect ratio (maximum diameter/minimum diameter) and are presented as means ± se; n = 12 for the blank; n = 12 for nonacclimated samples; and n = 6 for cold-acclimated samples.