FIG. 7.

Activation of MAP kinase and JNK upon Tat stimulation. (A) KS cells were stimulated with Tat (100 ng/ml) and immunoprecipitated with ERK-1 or ERK-2 antibody and then subjected to an in vitro kinase assay with MBP (7 μg) as a substrate. (B) Stimulated KS cells were immunoprecipitated with JNK antibody and subjected to an in vitro kinase assay with GST–c-Jun (1 to 79 amino acids) as the substrate. The ³²P-labeled proteins were subjected to SDS-PAGE (12% polyacrylamide) followed by autoradiography. Control lane represents immunoprecipitates of antibody alone.