Table I.
Oligonucleotides used in this study
Underlines indicate restriction sites.
| No. | Oligonucleotide Name | Sequence (5′-3′) |
|---|---|---|
| For construction of GST-PpRPOT1 fusion | ||
| 1 | 1GST.F | atggatccagatcgtcgtctgattctgtg |
| 2 | 1GST.R | atgtcgacaatgcactcatgacagcagg |
| For construction of GST-PpRPOT2 fusion | ||
| 3 | 2GST.F | atagatctttgacaccattagattccgca |
| 4 | 2GST.R | atgtcgacggcattaccatatgcttgac |
| For construction of PpRPOT1-GFP fusions | ||
| 5 | 1-GFP.R | atccatggagaatccaactttagtgt |
| 6 | 1M2.F | atgtcgacgtagcaatcggtgtgttgga |
| 7 | RBCS-RPOT1M1.F | atgtcgacttcatacagaagtgagaaaaatggtagcaatcggtgtgttggaac |
| 8 | RBCS-RPOT1M2.F | atgtcgacttcatacagaagtgagaaaaatgtggagggcggcagtaagg |
| 9 | 1M48I.F | gtgtgagaggcggaatctggagggcggc |
| 10 | 1M48I.R | gccgccctccagattccgcctctcacac |
| 11 | 1M1+1.F | atgtcgacatggtagcaatcggtgtgtt |
| 12 | 1M1-10.F | atgtcgactgaatcgtgcatggtagcaa |
| For construction of PpRPOT2-GFP fusions | ||
| 13 | 2-GFP.R | taccatggtcaaggaggaagggga |
| 14 | 2M2.F | atgtcgacccagctgaggtctgctggac |
| 15 | RBCS-RPOT2M1.F | atgtcgacttcatacagaagtgagaaaaatgccagctgaggtctgctggacga |
| 16 | RBCS-RPOT2M2.F | atgtcgacttcatacagaagtgagaaaaatgtggaggtcggcagcacag |
| 17 | 2M36I.F | cagtcgccggcatctggaggtcggcag |
| 18 | 2M36I.R | ctgccgacctccagatgccggcgactg |
| For construction of AtRpoT;2-GFP fusions | ||
| 19 | At2GFP.F | atgtcgacgacatgtgagaaacagagacaaccc |
| 20 | At2+1GFP.F | atgtcgacatgtccagtgctcaaacccc |
| 21 | At2GFP.R | atccatggcctcttcggctacactcgtgtac |
| For construction of GUS fusions | ||
| 22 | 1M1GUS.F | ctcgcgaaaatgaatcgtgcatgttacgtcctgtagaaac |
| 23 | 1M1.R | gcacgattcattttcgcgag |
| 24 | 1M2GUS.F | gcggtcgtgtgagaggcggaatgttacgtcctgtagaaac |
| 25 | 1M2.R | tccgcctctcacacgaccgc |
| 26 | 2M1-GUS.F | gcagggaattttaggggacaatgttacgtcctgtagaaac |
| 27 | 2M1.R | tgtcccctaaaattccctgc |
| 28 | 2M2-GUS.F | ccggttatccagtcgccggcatgttacgtcctgtagaaac |
| 29 | 2M2.R | gccggcgactggataaccgg |
| 30 | RBCS-GUS.F | atgtcgacttcatacagaagtgagaaaaatgttacgtcctgtagaaac |
| 31 | GUS.R | atgcggccgctcattgtttgcctccctgctgcgg |
| 32 | BcaBEST Sequencing Primer M13-20 | cgacgttgtaaaacgacggccagt |