Table II.

Effects of the 5′-upstream sequences on translation efficiency

GUS activity in P. patens protoplasts that were transformed with respective plasmid is normalized with the coexpressed GFP. The GUS values are expressed in arbitrary unit, with setting the average values of expression driven by the TL-GUS as 100. Each value (±se) represents the average of three independent assays. N/A, Not applicable.

Construct	Relative GUS Activity	P Value
Experiment 1
PpRPOT1u1-GUS	0.85 ± 0.34	0.51
PpRPOT1u2-GUS	93.03 ± 21.85	0.02
TL-GUS	100 ± 24.77	0.02
pUC18	0.67 ± 0.21	N/A
Experiment 2
PpRPOT2u1-GUS	1.10 ± 0.04	0.76
PpRPOT2u2-GUS	12.40 ± 0.44	<0.01
TL-GUS	100 ± 9.75	<0.01
pUC18	1.18 ± 0.09	N/A