Figure 3: Presence of phospho-mimicking Ser305Glu modifies the ability of pseudo-acetyl 5K-Q tau to be differentially seeded by AD-tau and PSP-tau seeds.

a. Linearized depiction of pseudo-acetyl 5K-Q tau carrying the phosphorylation-mimetic substitution on Ser305 (Ser→Glu) generated on human 0N/4R P301L mutant tau. b-d. Seeded HEK293T cells were fractionated into detergent-soluble and detergent-insoluble lysates and probed for total tau (t-tau) and p-tau (AT8). Representative immunoblots and % tau aggregation are shown. N=3 for each experimental replicate. Relative molecular masses (kDa) are indicated on the left of each blot. 2-way ANOVA with Dunnett’s multiple comparisons test, with single pooled variance. *p<0.05, **p<0.01.