Figure 4: Phosphorylation profile of seeded acetyl-substituted tau variants.

a. Schematic depiction of acetyl-mimetic 0N/4R P301L tau variants with antibody epitopes depicted. Depiction not to scale. b. The detergent-insoluble fractions of 5K-Q, 5K-R, 5K-Q/S305E and parent P301L tau variants seeded with AD:Patient A tau seeds or PSP:Patient A tau seeds, or unseeded were immunoblotted and probed with different p-tau epitopes PHF1 (pSer396/404), AT8 (pSer202/pThr205), AT270 (pThr181), and AT180 (Thr231). Representative blot from n=2 replicates.