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[Preprint]. 2024 Apr 15:2024.04.12.589253. [Version 1] doi: 10.1101/2024.04.12.589253

Figure 5: Comparative microtubule binding properties of acetyl-substituted tau.

Figure 5:

HEK293T cell-based microtubule binding assay was performed in the presence of Paclitaxel with cells transfected to express different P301L tau with acetyl-substituted epitopes, with GFP and no-DNA control. Antibody specific for β-tubulin was used to assess the microtubule polymerization under the same conditions. a-b. Quantification of microtubule-binding efficiency of 5K-Q, 5K-R, 5K-A compared to parent P301L tau. c-d. Quantification of microtubule-binding efficiency of K311Q, K353Q, K369Q, K370Q, K375Q compared to parent P301L tau. e-f. Quantification of microtubule-binding efficiency of 5K-Q/S305E mimetic compared to 5K-Q and parent P301L tau. The relative molecular masses of protein markers are indicated on the left. N=3 for each experimental replicate. 1-way ANOVA with Dunnett’s multiple comparisons test, with single pooled variance. *p<0.05, ****p<0.0001. S= supernatant fraction; P= pellet fraction. Data from microtubule binding assay without Paclitaxel treatment are shown in Fig. S3.