Figure 1. Bloom syndrome helicase (Blm) predicted structural order and alleles used.

(A) IUPred3 (2) plot predicting the ordered and intrinsically disordered regions of Blm. A schematic of Blm domains is placed below for reference, illustrating that conserved regions 1 and 2 (CR1 and CR2) are predicted to be mostly disordered (>0.5). (B) Previously characterized alleles of Blm and their effects on Blm functions relative to wild-type (wt). A null allele, Blm^N1 (N1) eliminates all well-characterized Blm functions, while the separation-of-function allele Blm^N2 (N2) only moderately affects embryonic development. (C) Schematic of Blm deletions used compared to the wt Blm protein. ΔCR1 deletes amino acids 2–240, preserving amino acids 241–1487 in frame; ΔCR2 deletes amino acids 576–720, fusing amino acids 1–575 and 721–1487 together in frame.