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[Preprint]. 2024 Apr 15:2024.04.12.589165. [Version 1] doi: 10.1101/2024.04.12.589165

Figure 3. Meiotic non-disjunction (NDJ).

Figure 3.

Virgin females with the Blm alleles indicated on the X-axis over the BlmN1 null allele (BlmN1 was over BlmD2) were crossed to y sc cv v g f / Dp(1;Y)BS males in at least 15 vials, each serving as a biological replicate. Progeny were scored for non-disjunction (NDJ), indicated by bar eyes in daughters (XXY) and non-bar-eyes in sons (X0) genotypes. The number of NDJ progeny was doubled to correct for genotypes that do not progress to adulthood (XXX and Y0), then NDJ rate was determined as a ratio of the number of corrected NDJ individuals to total progeny for each genotype. Neither BlmΔCR1 (ΔCR1) nor BlmΔCR2 (ΔCR2) had a significant increase in NDJ compared to wild type (wt). In agreement with prior studied, both BlmN1 and BlmN2 females have significantly elevated NDJ. Number of progeny = wt: 6900; ΔCR1: 3593; ΔCR2: 777; N1: 2959; N2: 1906. **** p < 0.0001; ns: p > 0.05 by the methods described in Zeng et al. (4).