Figure 5. Repair of DNA gaps by SDSA.

Single males with the Blm alleles indicated on the X-axis (in trans to a Blm^D2 null allele) and the Δ2–3 transposase were crossed to homozygous P{w^a} virgin females, with each vial serving as a biological replicate. Progeny without the Δ2–3 transposase were scored for the type of repair that occurred in the parental male’s germline, with red eyes indicating completed SDSA, yellow or white eyes indicating end-joining, and apricot eyes indicating either no excision or repair that restored the complete P{w^a}. SDSA frequency is the percentage of proteny with red eyes. All mutants had significantly lower numbers of red-eyed progeny than wild-type. ****p<0.0001 by ANOVA with Tukey’s Post Hoc and Kruskal-Wallis with Dunn’s Multiple comparisons. n =wt: 151 vials, 4675 progeny; ΔCR1: 45 vials, 6393 progeny; ΔCR2: 148 vials, 4328 progeny; N1: 106 vials, 4197 progeny; N2: 133 vials, 3860 progeny.