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[Preprint]. 2024 Apr 16:2024.04.16.589782. [Version 1] doi: 10.1101/2024.04.16.589782

Fig. 4. Presence of synaptopodin and spine apparatus in Drosophila melanogaster.

Fig. 4

A. Residues 464–508 of mouse synaptopodin (calsarcin domain) are highly conserved among synaptopodin orthologues in vertebrates and D. melanogaster orthologue, CG1674. B. COS-7 cell expressing the fluorescently tagged D. melanogaster synaptopodin orthologue. A prominent accumulation of CG1674 inclusions, similar to those observed in cells expressing fluorescently tagged mouse synaptopodin (See Fig. 2 and 3C), is visible. The CG1674 signal is shown in gray scale (left), and color-coded based on distance from coverslip (right). C. D. melanogaster ‘s LPTC neuron reconstructed from 3D SBF-SEM images (from FlyWire [46]) showing spines along its major process. Low and high magnification views are shown at the top and bottom, respectively. D. SBF-SEM sequential optical sections from a spine of the neurons shown in C, revealing ER elements closely apposed via an intervening density (orange arrows) in the spine head. This structure is reminiscent of a spine apparatus with dilated ER cisterns, possibly due to preparation artifacts. E. Another example of a dendritic spine of D. melanogaster containing a structure reminiscent of a spine apparatus, but with a dilated ER.