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[Preprint]. 2024 Apr 15:2024.04.15.589443. [Version 1] doi: 10.1101/2024.04.15.589443

Figure 4: Stability and Degradation of RecA Splicing Products.

Figure 4:

(A,B) Mid-log Mtb cultures were treated with Mitomycin C (MMC) for 24 hours, then treated with chloramphenicol (CAM) for the indicated times to block new protein synthesis. (A) Western blot analysis of intein levels over time. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP. (B) Western blot analysis of ligated extein levels over time. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP. (C,D) Mtb cultures were treated (+) with bortezomib (BTZ) to block proteasome activity or left untreated (−). (C) Western blot analysis of intein levels over time. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP. (D) Western blot analysis of ligated extein, LE, NEA, and NE levels after BTZ treatment. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP; ** indicates p < 0.01.