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[Preprint]. 2024 Apr 12:rs.3.rs-4078220. [Version 1] doi: 10.21203/rs.3.rs-4078220/v1

Figure 6: Physical differences in tissue explant.

Figure 6:

a) This bar chart shows the difference in settled TEER value of tissues explants with the muscle intact vs. with the muscle removed. The settled TEER value is taken 24 hours after the tissue is enclosed in the chamber. i) Tol blue staining of tissue with intact muscle. ii) Tol blue staining tissue with muscle removed, L = lumen, scale bars = 50μm. b) Settled TEER values for different regions of mouse colon tissue. Proximal tissue was defined as the three pieces of tissue closest to the cecum and distal tissue was defined as the two pieces farthest from the cecum and closest to the rectum. Each tissue piece was approximately 5mm in length. The settled TEER value was taken approximately 24 hours after the tissue had been enclosed in the device. Muscle intact: n = 15, Cut muscle: n = 7, Distal: n = 6, Proximal: n = 9, **: P < 0.005, *: P < 0.01.